例1)scFv製造 - ハイブリドーマセルラインのシーケンシングからの発現ベクターまで

           以下の価格はすべて参考価格です。ライセンス事案の場合等、都度見積もりをお出しいたします。

 

1. Vh and Vl sequencing from hybridoma cell line

We charge ¥350,000 to sequence the Vh and Vl region from hybridoma cell line.

 

2. Construction of the scFv expression construct

We charge ¥205,000/ea to construct an expression vector for the scFv antibody, covering below process:

 

    • Gene synthesis plus codon optimization, there are two genes, Vh and Vl, ¥3,000/ea, ¥58,000in total
    • Design and construct the scFv construct. Please note that there are two different orientations for one scFv antibody as below. It's hard to predict which one works better. And it's even possible that one orientation is no longer functional. We highly recommend to try both orientations. For each orientation construct, the price is ¥73,000.
      • N'-Vh-linker-Vl-C'
      • N'-Vl-linker-Vh-N'
    • Clone the scFv construct into the expression vector. Validate the gene by DNA sequencing.¥80,000/ea.

In summary, if you want to get both scFv constructs with different orientations, the sum is ¥350,000. We just charge for the gene synthesis for once. If you want just one scFv construct, please assign the orientation and your price is¥205,000.