Premade Phage Display Peptide Libraries

http://www.creative-biolabs.com/images/Phage-Peptide-Library.png

Besides using the publically available peptide libraries, Creative Biolabs also provide 3 peptide libraries that were constructed in house:

http://www.creative-biolabs.com/images/icon_02.gif TriCo-16TM Phage Display Peptide Library is a pIII-fusion16-mer random peptide library based on a true phage vector. The random peptides are encoded by DNA sequences that were randomized using Trinucleotide Phosphoramidites (Trimer Codons) of bacterial preference, thus completely avoiding stop and non-sense codons. In addition, the trimer codon for cysteine was excluded. The current size of this library is 2.60×1010.

http://www.creative-biolabs.com/images/icon_02.gif TriCo-20TM Phage Display Peptide Library is a pIII-fusion20-mer random peptide library based on a true phage vector. The random peptides are encoded by DNA sequences that were randomized using Trinucleotide Phosphoramidites (Trimer Codons) of bacterial preference, thus completely avoiding stop and non-sense codons. In addition, the trimer codon for cysteine was excluded. The current size of this library is 1.70×109.

As validated by quality control DNA sequencing, more than 97% of randomly picked clones from these two libraries contained dsDNA inserts, and only an insignificant percentage of them harbored longer or shorter diversified peptides. None stop codons were founded as expected. Also, there was no single nucleotide deletion or insertion detected; importantly, non-sense frame-shift mutation was completely absent, demonstrating the beauty of trimer-codon strategy. Moreover, no Cys encoding codon was identified in the peptide library as designed. These Random Peptide Libraries are the best random 16-mer and 20-mer peptide libraries in terms of their quality and diversity.

http://www.creative-biolabs.com/images/icon_02.gif Phage Display Peptide-10TM Library is a pIII-fusion 10-mer peptide library constructed in a phagemid vector using NNK method with a size of 1.2×109.

Creative Biolabs also provide the following specific peptide libraries:

Besides using the publically available peptide libraries, Creative Biolabs also provide 3 peptide libraries that were constructed in house:  

                                             価格はお問い合わせください。都度お見積もりいたします。

 

Libraries

Type

Length

Library Size

Price

Peptide-10TM

pIII-fusion

10-mer peptide library

1.0x109

F88-4

pIII-fusion

15-mer peptide library

2.0x109

F88-Cys1

pVIII-fusion (~150 copies)

10-mer peptide library

5.6x108

F88-Cys2

pVIII-fusion (~150 copies)

11-mer peptide library

2.8x109

F88-Cys3

pVIII-fusion (~150 copies)

12-mer peptide library

5.5x107

F88-Cys4

pVIII-fusion (~150 copies)

12-mer peptide library

5.5x107

F88-Cys5

pVIII-fusion (~150 copies)

12-mer peptide library

5.9x108

F88-Cys6

pVIII-fusion (~150 copies)

14-mer peptide library

2.7x108

FUSE5-6

pIII-fusion

6-mer peptide library

2.0x108

FUSE5-15

pIII-fusion

15-mer peptide library

2.5x108

 

                       株式会社 バイオロジカ

                        biologic@rr.iij4u.or.jp

                                                            Return