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InstantAgaroseTablet Gel Preparation Protocol

 

product

Instant Agarose

CAS

 

appearance

White to off-white

EEO

≤ 0.13

Gelling point

36‹C}1.5‹C

(1.5% gel)

Melting point

88‹C}1.5‹C 

(1.5% gel) 

solubility

1g in 100ml water

moisture

  ≤ 10%

Gel strength

≥ 1200 g/cm2 

(1% Gel) 

Sulfate

≤ 0.15%

Ash

≤ 0.5%

Impurities

None Detected

 

Product: InstantAgarose™ Tablet, 0.5g@

8,000/200 tab

 

1)    Use a flask that is 2 to 4 times the volume of the solution being prepared.IMPORTANT

2)    Add agarose tablet to electrophoresis buffer based on below   

table to prepare your desired gel %, soak tablet in buffer for   

2 minutes before heating.

3) If use boiling water bath:

To melt agarose, simply heat the slurry in a boiling water  

bath, bring the solution to a boil and allow it to boil for 5-10

minutes stirring continuously, until agarose dissolves

completely.

If use microwave oven:

Heat the slurry in microwave on high power setting until it

starts to boil, allow boiling for 30 seconds.

Remove the flask from microwave, swirl gently to re-suspend

any remaining agarose particles.

Reheat on high power for 1-2 minutes or until the solution is clear and all particles are dissolved.

Remove the flask from the microwave oven, and gently swirl to it.

Use caution when handling as solution may be extremely heated.

4) Cool the solution to approx. 60‹C before pouring.

Separation of DNA in agarose

 

 

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