since 1984

 

siRNA合成サービス:DNA, RNA, ペプチド合成のパイオニア、30年余の実績があります

Biosynthesis - Biologica 25年の実績

 

Custom siRNA Synthesis - Road to gene silencing

Our custom siRNA synthesis services are delivered in desalted, purified, annealed duplex format with modified nucleotides of DNA , 2'OMe, and Constrained Nucleotide bases. Provide your siRNA sequence of choice, or use our design tool to select a siRNA sequence. siRNAduplexes are provided as a lyophilized pellet to ensure stability during delivery. siRNA duplex are available from milligram to gram scale both as chemically stabilized and as unmodified RNA. Large-scale sterile, endotoxin-free preparations are available specifically for in vivo research needs. 

 

Duplex siRNA - 21-mer

Our siRNA oligos are available at a fixed price per oligonucleotide which are made of 19 RNA bases plus 2 DNA bases 3' overhang. 

Contact us for longer oligos or spike oligos which are available upon requests.

 

Standard

HPLC

PAGE

In vivo

In vivo HPLC

 

Purified

Purified

 

Purified

Desalted

Desalted

Desalted

Desalted

Desalted

Duplex

Duplexed

Duplexed

Duplexed

Duplexed

 

For modified

RNA

For modified

RNA

Endtoxin tested

Endtoxin tested

 

 

 

Na+Salt Exchange

Na+Salt Exchange

 

 

 

In vivo use

modified  siRNA

In vivo use

20 nmol

48,000

 

75,000

 

75,000

25 nmol

112,000

50 nmol

165,000

40 nmol

65,000

 

104,000

 

113,000

100 nmol

124,000

100 nmol

204,000

150 nmol

76,000

75 nmol

114,000

80 nmol

120,000

200 nmol

162,000

250 nmol

270,000

 300 nmol

114,000

 150 nmol

152,000

150 nmol

162,000

 500 nmol

217,000

500 nmol

417,000

 

 

                                                             より大きなスケールの供給も致します。お問い合わせください。

                  直接納入の場合、10%引きとなります。10万円以上の場合、15%引き

*Guaranteed yields (ODs) and approximate yields (nmols) are for oligos > 20 bases and < 40 bases *

 

Duplex siRNA - 27-mer

These siRNA molecules have no dTdT overhang or any sticky overhang at all. 27-mers might show higher efficiency in comparison to the 'classical' 21-mer siRNA in certain cases (Kim et al. Nature Biotechnology 2005 23 (2):222-226).

 

Standard

HPLC

PAGE

In vivo

In vivo HPLC

 

Purified

Purified

 

Purified

Desalted

Desalted

Desalted

Desalted

Desalted

Duplex

Duplexed

Duplexed

Duplexed

Duplexed

 

For modified

RNA

For modified

RNA

Endtoxin tested

Endtoxin tested

 

 

 

Na+Salt Exchange

Na+Salt Exchange

 

 

 

In vivo use

r modified  siRNA

In vivo use

20 nmol

75,000

 

95,000

15 nmol

95,000

25 nmol

143,000

50 nmol

213,000

40 nmol

84,000

 

133,000

30 nmol

145,000

100 nmol

159,000

100 nmol

262,000

150 nmol

97,000

75 nmol

147,000

65 nmol

154,000

200 nmol

208,000

250 nmol

347,000

 300 nmol

148,000

 150 nmol

196,000

120 nmol

208,000

 500 nmol

279,000

500 nmol

535,000

 

 

より大きなスケールの供給も致します。お問い合わせください。

                 直接納入の場合、10%引きとなります。10万円以上の場合、15%引き

*Guaranteed yields (ODs) and approximate yields (nmols) are for oligos > 20 bases and < 40 bases *

 

 

Sample Processing Options

o    Desalted Single-Stranded:

  1. The oligos are shipped in the deprotected, desalted single strand in a separate tube.
  2. The dried duplex is provided in the absence of universal buffer salts.
  3. The oligos are ready for use immediately upon resuspension.
  4. The expected ship date is 4 business days for orders received before 3:00pm CST.

o    Desalted Duplex:

  1. The oligos are shipped in the deprotected, desalted duplex form.
  2. The dried duplex is provided in the absence of universal buffer salts.
  3. The oligos are ready for use immediately upon resuspension.
  4. The expected ship date is 4 business days for orders received before 3:00pm CST.

o    HPLC purified: *Special Note: The siRNA duplex is now dried down in the absence of buffer and the amount packaged per tube has changed, however, the total amount of material supplied remains the same.

  1. The oligos are shipped in the deprotected, duplexed, purified, and desalted form.
  2. The dried duplex is provided in the absence of universal buffer salts, unless otherwise specified upon ordering.
  3. The oligos are ready for use immediately upon resuspension.
  4. The expected ship date is 6 business days for orders received before 3:00pm CST.

o    siRNA In-Vivo-Ready: A minimum of 90% purity. These duplexes are shipped in the deprotected form with extra processing also including counter ion (Na+) exchange, dialysis, sterile filtration and endotoxin testing

 for the introduction of siRNAs into animals.

The estimated ship date is 10 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.

o    HPLC purified: This In-Vivo-Ready custom synthesized siRNAs provide the highest quality siRNAs available anywhere with guaranteed purity of 97%. The processing of the oligos includes extra level of HPLC purification, counter ion (Na+) exchange, dialysis to remove salt (to <1 microsiemens conductance at 50 µM siRNA), sterile filtration and endotoxin tested.

The estimated ship date is 20 business days for orders received before 3:00pm CST. Ship date will be confirmed upon order placement.

 

Quality Control Options

 

Standard QC package

Analytical anion-exchange HPLC of oligonucleotide; purity guaranteed above 85% in case purification is required. TOF/MS for oligonucleotide identification.

Advanced QC package

Standard QC package and additionally:

Ion-pair reverse phase HPLC of the oligonucleotide at 60oC, purity guaranteed above 85% analytical reverse phase HPLC Endotoxin analysis of the oligonucleotide (*phosphorothioates may inhibit in the mechanism for endotoxin determinations Bio- Synthesis is using and therefore reading might be less accurate for these molecules) pH determination of a 1% oligonucleotide solution.

Custom QC Package: Custom QC package such as:

Determination of the sodium content of the oligonucleotide (minimum 20 mg of purified oligonucleotide is required). LC-MS of the oligonucleotides. Any custom quality control methods could be developed. Our technical service staffs are willing to respond to your various advices and requests.

 

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株式会社 バイオロジカ
名古屋市中区大須4−10−40
    052-262-6732, biologic@rr.iij4u.or.jp