ChunLab NGS Service Price List

 

 

Microbiome (16S /ITS gene) Full Service    

 

Service includes:  

 DNA sample QC

 Library construction

 Roche 454 or MiSeq 16S amplicon sequencing

 Comprehensive data analysis

 Creation of CLC format files to be browsed and further analyzed in ChunLab’s CLcommunity software

CLcommunity software included free  

 

 

 

10 or less samples

11-20

21-50

454 Junior, 5K reads

26,000/sample

¥22,800

¥21,500

MiSeq Nano Flow Cell

(250bp PE), 20K reads

21,000/sample

19,500

17,600

                                       

 

RNA Sequencing  Full Service    

 

Service includes:

 Sample QC

 Strand specific library construction (rRNA depletion for Bacterial samples only)

Illumina HiSeq 2500 sequencing

 Comprehensive data analysis 

 Creation of CLT and CLG format files to be browsed and further analyzed in ChunLab’s CLRNASeq software

CLRNASeq software included free 

 

 

 

or less samples

512

13

Bacteria, 50bp SE

¥156,000/sample

¥148,000

¥130,000

Eukaryote, 100bp PE

169,000/sample

163,000

149,000

 

 

Bacterial Genome De novo Sequencing and Data Analysis  

 

Service includes:

 Sample QC

MiSeq 300bp PE sequencing; >100X coverage

PacBio C4-P6, >70X coverage

 Comprehensive data analysis

 Creation of CLG and CG format files to be browsed and further analyzed in ChunLab’s CLgenomics software 

CLgenomics software included free   

 

 

MiSeq 300bp PE sequencing; >100X coverage

¥156,000

PacBio C4-P6, >70X coverage

364,000

Resequencing     MiSeq

117,000

 

 

Bioinformatics  Only Services

Microbiome (16S /ITS gene) Bioinformatics  Analysis with Software    

 

Analysis includes:

 Superior phylogenetic identification and taxonomic assignment of each read at the species level based on the EzTaxon and EzFungi databases

 Alpha and beta diversity indices, rarefaction curves, rank abundance, environmental correlations, source tracking, clustering, UniFrac multivariate analysis, canonical correspondence analysis

 Creation of CLC format files to be browsed and further analyzed in ChunLab’s CLcommunity software

CLcommunity software included free 

Analysis for any platform  Analysis not possible if LT 16S metagenomic kit is used    

 

 

 

<10 samples

11-20

21-50

51-100

101-250

>250

<50K

¥13,000/sample

¥11,000

¥7,800

¥6,500

¥5,200

¥3,900

51-100K

¥15,000/sample

13,000

9,800

7,800

6,500

4,600

101-200K

¥17,600/sample

15,000

11,000

9,800

7,800

5,900

201K-300K

¥20,200/sample

17,600

13,000

11,700

9,100

7,200

 

 

RNA Sequencing Bioinformatics Analysis with Software    

 

Analysis includes:

 Mapping of transcript expressions (of multiple samples) to reference genome of client’s choice 

 Differential gene expression analysis between any two selected samples (DEG Browser)

 KEGG, eggnog and gene ontology metabolic and pathway enrichment analyses

 Clustering of gene groups with similar expression patterns

 SNP / InDel detection

 Creation of CLT format files to be browsed and further analyzed in ChunLab’s CLRNASeq software

CLRNASeq software included free       

 

 

1~3

4~9

10~29

30~49

50~100

¥31,200/sample

29,300

26,000

22,800

19,500

 

 

Microbial Genome Analysis Bioinformatics Only  

 

Analysis Includes:

 Assembly

 Annotation (SEED, RefSeq, Subsystems, COG and KEGG)

 Plus 3 comparative genome analysis (gene by gene level comparison)

 Creation of CLG and CG format files to be browsed and further analyzed in ChunLab’s CLgenomics software

CLgenomics software included free 

De novo (genome size up to 5MB)

¥39,000 for assembly*

¥52,000 for annotation

¥39,000 SNV/SNP Analysis (analysis provided in Excel) *assembly price increase by ¥13,000per 1MB  

Comparative Genomics

¥26,000 for 3 genomes  ¥39,000 for 5 genomes  ¥65,000 for 10 genomes  ¥130,000 for 20 genomes  

Non NGS Services Description Pricing  

 

Bionano Irys Map Service  

 

Service Includes:

 DNA Plug QC

 Library Creation

 Mapping using Irys Chip

Irys View Software for viewing map, uploading NGS data for alignment and scaffolding, and comparison on different Irys maps 

One Irys chip contains 2 lanes One lane produces 10-50GB of data 

Component pricing

¥11,700 – Plug QC

¥70,000 – library

¥320,000  per chip *As plug preparation is tedious, recommend clients to submit 5 plugs at one time **Coverage of 50X is minimum but recommend 100X for best results 

 

nCounter  Digital Count Profiling 

 

Service Includes:

 Sample QC

nCounter Analysis

 Data analysis – results delivered in Excel / Report format  

 

 

株式会社 バイオロジカ

名古屋市中区大須4−10−40

o52-262-6732

biologic@rr.iij4u.or.jp